Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence.

Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence† †Electronic supplementary information (ESI) available: General procedures, Tb–Xo4 synthesis, Fig. S1–S11 and Tables S1–S4. See DOI: 10.1039/c7sc00758b

Lanthanide-tagged proteins--an illuminating partnership.

Lanthanide-tagged proteins are valuable for exploiting the unique properties of Ln ions for investigating protein structure, function, and dynamics. Introduction of the Ln into the target is accomplished via chemical modification with synthetic lanthanide-chelating prosthetic groups or by coexpression with peptide-based binding tags. Complexed Ln-tags offer a heavy-atom site for solving the pha...

A new class of lanthanide complexes to obtain high-phasing-power heavy-atom derivatives for macromolecular crystallography.

Because of their intense white lines and large f" values, lanthanide atoms are of great interest for solving structures of biological macromolecules using single-wavelength anomalous diffraction (SAD) or multiple-wavelength anomalous diffraction (MAD) methods. In this work, a series of seven gadolinium complexes are described which provide excellent derivatives for anomalous diffraction experim...

Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.

We recently engineered encodable lanthanide binding tags (LBTs) into proteins and demonstrated their applicability in Nuclear Magnetic Resonance (NMR) spectroscopy, X-ray crystallography and luminescence studies. Here, we engineered two-loop-LBTs into the model protein interleukin-1β (IL1β) and measured (1)H, (15)N-pseudocontact shifts (PCSs) by NMR spectroscopy. We determined the Δχ-tensors as...

Thiol-reactive lanthanide chelates for phasing protein X-ray diffraction data.

Lanthanides can contribute a large anomalous component to X-ray scattering when present and ordered in a target crystal. This large anomalous signal is a useful source of phase information in X-ray crystallographic studies of biological macromolecules. Thiol-reactive lanthanide chelates were tested as a means of incorporation of lanthanides into protein crystals. Two compounds, each capable of ...